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Using an on-site laboratory for fecal steroid analysis in wild white-faced capuchins

CSS Publication Number
CSS22-53
Full Publication Date
August 26, 2022
Abstract

Hormone laboratories located “on-site” where field studies are being conducted have a number of advantages. On-site laboratories allow hormone analyses to proceed in near-real-time, minimize logistics of sample permits/shipping, contribute to in-country capacity-building, and (our focus here) facilitate cross-site collaboration through shared methods and a shared laboratory. Here we provide proof-of-concept that an on-site hormone laboratory (the Taboga Field Laboratory, located in the Taboga Forest Reserve, Costa Rica) can successfully run endocrine analyses in a remote location. Using fecal samples from wild white-faced capuchins (Cebus imitator) from three Costa Rican forests, we validate the extraction and analysis of four steroid hormones (glucocorticoids, testosterone, estradiol, progesterone) across six assays (DetectX® and ISWE, all from Arbor Assays). Additionally, as the first collaboration across three long-term, wild capuchin field sites (Lomas Barbudal, Santa Rosa, Taboga) involving local Costa Rican collaborators, this laboratory can serve as a future hub for collaborative exchange.

Co-Author(s)
Jacinta C. Beehner
Cloe Allen
Marcela E. Benítez
Thore J. Bergman
Margaret S. Buehler
Sofia C. Carrera
Emily M. Chester
Tobias Deschner
Alexander Fuentes
Colleen M. Gault
Irene Godoy
Katharine M. Jack
Justin D. Kim
Lev Kolinski
Nelle K. Kulick
Teera Losch
Juan Carlos Ordoñez
Susan E. Perry
Fernando Pinto
Olivia T. Reilly
Elizabeth Tinsley Johnson
Michael D. Wasserman
Keywords

Androgens

Cortisol

Estradiol

Estrogens

Field laboratory

Glucocorticoids

Progesterone

Progestogens

Testosterone

Validation

Publication Type
Journal Article
Digital Object Identifier
https://doi.org/10.1016/j.ygcen.2022.114109
Full Citation

Jacinta C. Beehner, José Alfaro, Cloe Allen, Marcela E. Benítez, Thore J. Bergman, Margaret S. Buehler, Sofia C. Carrera, Emily M. Chester, Tobias Deschner, Alexander Fuentes, Colleen M. Gault, Irene Godoy, Katharine M. Jack, Justin D. Kim, Lev Kolinski, Nelle K. Kulick, Teera Losch, Juan Carlos Ordoñez, Susan E. Perry, Fernando Pinto, Olivia T. Reilly, Elizabeth Tinsley Johnson, Michael D. Wasserman, Using an on-site laboratory for fecal steroid analysis in wild white-faced capuchins, General and Comparative Endocrinology, Volume 329, 2022. CSS22-53